In vitro gene silencing of independent phosphoglycerate ...

2013-3-25  In vitro gene silencing of independent phosphoglycerate mutase (iPGM) in the filarial parasite Brugia malayi. Singh PK(1), Kushwaha S, Mohd S, Pathak M, Misra-Bhattacharya S. Author information: (1)Division of Parasitology, CSIR-Central Drug Research Institute, B,S, 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow, UP, 226021, India ...

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In vitro gene silencing of independent phosphoglycerate ...

2013-3-25  The in vitro ipgmgene silencing by small interfering RNA (siRNA) leads to severe phenotypic deformities in the intrauterine developmental stages of female worms with a drastic reduction (~90%) in the motility of adult parasites and a significantly reduced (80%)

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In vitro gene silencing of independent phosphoglycerate ...

2013-3-25  The Bm-ipgm gene silencing in the infective L3 reduces their motility and impairs their further development in jirds. In vitro silencing of the Bm-ipgm gene in the L3 brought about a reduction in the motility of almost half the cultured L3 at 48 hours in contrast to the control group, where 10-12% of the L3 became sluggish. Under normal culture ...

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RESEARCH ARTICLE Open Access In vitro gene silencing

2017-8-25  Results: The in vitro ipgm gene silencing by small interfering RNA (siRNA) leads to severe phenotypic deformities in the intrauterine developmental stages of female worms with a drastic reduction (~90%) in the motility of adult parasites and a significantly reduced (80%) release of microfilariae (mf) by female worms in vitro.

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(PDF) In vitro gene silencing of independent ...

2013-3-21  In vitro gene silencing of independent phosphoglycerate mutase (iPGM) in the filarial parasite Brugia malayi.pdf Available via license: CC BY 2.0 Content may be subject to copyright.

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In vitro gene silencing of independent phosphoglycerate ...

In vitro gene silencing of independent phosphoglycerate mutase (iPGM) in the filarial parasite Brugia malayi ระบบคลังข้อมูลงานวิจัยไทยโดยคณะทำงานเพื่อพัฒนาและบูรณาการระบบข้อมูลวิจัยของประเทศ พ.ศ. 2554 อันประกอบ ...

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Gene silencing during development of in vitro-produced ...

2012-10-26  duced in vitro stray significantly from a 1:1 sex ratio (Nicol et al., 2000; Iwata et al., 2002). The identification of modifications of X-related gene expression in the embryo can provide us with a means to better understand the effect of in vitro culture on the development of male and female embryos.

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In vivo gene silencing identifies the Mycobacterium ...

2007-12-2  Genes that are essential for growth in vitro, in vivo or both account for approximately 20% of Mtb 's genome. Conditional gene silencing could therefore facilitate the validation of up to 800...

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Engineering of small interfering RNA-loaded lipidoid-poly ...

2017-11-1  The optimized lipidoid-modified LPNs revealed more than 50-fold higher in vitro gene silencing at well-tolerated doses and approx. a twofold increase in siRNA loading as compared to reference LPNs modified with the commonly used cationic

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Reversal of siRNA-mediated gene silencing in vivo Nature ...

2018-5-14  Gene silencing by siRNAs in vivo is reversed using sequence-specific high-affinity oligonucleotides. We report rapid, potent reversal of GalNAc

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Methylation-independent silencing of the p73 gene in ...

p73 is a p53 homolog that, in vitro, inhibits cell growth and induce apoptosis. In some tumors p73 is monoallelically expressed and this raised the possibility that this gene is subjected to imprinting. Silencing of p73 in acute leukemia and in Burkitt's lymphoma occurs in association with the aberrant methylation of the first exon of the gene.

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Frontiers Host-Induced Gene Silencing of a Sclerotinia ...

2021-7-20  External Application of RNAs Targeting Ssoah1 Reduced Transcript Levels in vitro. We next tested whether the external application of dsRNAs leads to the silencing of the target gene. In three independent experiments, actively growing S. sclerotiorum hyphal plugs were placed in potato dextrose broth (PDB) containing in vitro transcribed Ssoah1-dsRNAs; GFP-dsRNA treatment served as a

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Id1 overexpression is independent of repression and ...

2020-4-8  Id1 overexpression is independent of repression and epigenetic silencing of tumor suppressor genes in melanoma ... genes were resistant to Id1-mediated gene silencing. Our results suggest that Id1 activation may need to occur at discrete ... in vitro methylated DNa (IVD) was a methylated control. Bh20 is bisulfite-treated no DNa control and h20 ...

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Gene silencing of indoleamine 2,3-dioxygenase 2 in ...

Gene silencing of indoleamine 2,3-dioxygenase 2 in melanoma ... is known about the immune-independent functions of IDO2 relevant to its specific ... and reduces cell migration in vitro.

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In Vitro Gene Silencing of the Fish Microsporidian ...

2019-9-9  This in vitro system has been used successfully as a model for screening and development of drugs and enables us to conduct RNA interference (RNAi) experiments . RNAi is a natural mechanism for posttranscriptional gene silencing triggered by small interfering RNA (siRNA).

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Engineering of small interfering RNA-loaded lipidoid-poly ...

2017-11-1  The in vitro gene silencing experiments were performed in duplicate. The in vitro gene silencing for three independent batches of a representative formulation is also provided as supplementary data : the standard deviation was generally below 10%. The cell viability experiments was performed in quadruplicate, and the standard deviation was also ...

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Prediction and in vitro silencing of two lipid ...

2020-10-21  Prediction and in vitro silencing of two lipid biosynthetic genes viz. SCD and SREBP1 in chicken using RNA interference N. Govardhana Sagar1, A. Rajendra Prasad1, R.N. Chatterjee2, Pushpendra Kumar1, Bharat Bhushan1, P. Guru Vishnu1 and T. K. Bhattacharya2* 1 ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P, India 2ICAR-Directorate of Poultry Research,

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Frontiers Host-Induced Gene Silencing of MoAP1 Confers ...

2019-4-9  Rice blast caused by Magnaporthe oryzae (M. oryzae) is a major threat to global rice production. In recent years, small interference RNAs (siRNAs) and host-induced gene silencing (HIGS) has been shown to be new strategies for the development of transgenic plants to control fungal diseases and proved a useful tool to study gene function in pathogens. We here tested whether in vitro

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Simultaneous silencing of two different Arabidopsis genes ...

2021-1-6  Virus-induced gene silencing (VIGS) is a useful tool for functional characterizations of plant genes. However, the penetrance of VIGS varies depending on the genes to be silenced, and has to be evaluated by examining the transcript levels of target genes. In this report, we report the development of a novel VIGS vector that permits a preliminary assessment of the silencing penetrance.

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Host‐induced gene silencing of multiple genes of

Brief Communication Host-induced gene silencing of multiple genes of Fusarium graminearum enhances resistance to Fusarium head blight in Gravel Minhui Wang1,2,†, Lei Wu3,†, Yuzhen Mei1,2, Youfu Zhao4, Zhonghua Ma1,2,*, Xu Zhang3,* and Yun Chen1,2,* 1Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China

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Methylation-independent silencing of the p73 gene in ...

p73 is a p53 homolog that, in vitro, inhibits cell growth and induce apoptosis. In some tumors p73 is monoallelically expressed and this raised the possibility that this gene is subjected to imprinting. Silencing of p73 in acute leukemia and in Burkitt's lymphoma occurs in association with the aberrant methylation of the first exon of the gene.

Get Price

Gene silencing during development of in vitro-produced ...

2012-10-26  duced in vitro stray significantly from a 1:1 sex ratio (Nicol et al., 2000; Iwata et al., 2002). The identification of modifications of X-related gene expression in the embryo can provide us with a means to better understand the effect of in vitro culture on the development of male and female embryos.

Get Price

Id1 overexpression is independent of repression and ...

2020-4-8  Id1 overexpression is independent of repression and epigenetic silencing of tumor suppressor genes in melanoma ... genes were resistant to Id1-mediated gene silencing. Our results suggest that Id1 activation may need to occur at discrete ... in vitro methylated DNa (IVD) was a methylated control. Bh20 is bisulfite-treated no DNa control and h20 ...

Get Price

Frontiers Host-Induced Gene Silencing of a Sclerotinia ...

2021-7-20  External Application of RNAs Targeting Ssoah1 Reduced Transcript Levels in vitro. We next tested whether the external application of dsRNAs leads to the silencing of the target gene. In three independent experiments, actively growing S. sclerotiorum hyphal plugs were placed in potato dextrose broth (PDB) containing in vitro transcribed Ssoah1-dsRNAs; GFP-dsRNA treatment served as a

Get Price

Gene silencing of indoleamine 2,3-dioxygenase 2 in ...

Gene silencing of indoleamine 2,3-dioxygenase 2 in melanoma ... is known about the immune-independent functions of IDO2 relevant to its specific ... and reduces cell migration in vitro.

Get Price

In Vitro Gene Silencing of the Fish Microsporidian ...

2019-9-9  This in vitro system has been used successfully as a model for screening and development of drugs and enables us to conduct RNA interference (RNAi) experiments . RNAi is a natural mechanism for posttranscriptional gene silencing triggered by small interfering RNA (siRNA).

Get Price

FKZ 2734 : In vitro gene silencing von Kollagenen in ...

2010-6-9  2734: In vitro gene silencing von Kollagenen in Nerven- und Gliazellen Figure 1: Analysis of knockdown efficiency of COL16A1 (A) Knockdown efficiency of COL16A1 was performed at mRNA level with quantitative PCR and shows ~60% lower COL16A1 gene expression in transfected cells compared to control cells which were transfected with scrambled RNA.

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Prediction and in vitro silencing of two lipid ...

2020-10-21  Prediction and in vitro silencing of two lipid biosynthetic genes viz. SCD and SREBP1 in chicken using RNA interference N. Govardhana Sagar1, A. Rajendra Prasad1, R.N. Chatterjee2, Pushpendra Kumar1, Bharat Bhushan1, P. Guru Vishnu1 and T. K. Bhattacharya2* 1 ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P, India 2ICAR-Directorate of Poultry Research,

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CRISPR-Cas9 Mediated Gene-Silencing of the Mutant ...

2019-2-22  CRISPR-Cas9 Mediated Gene-Silencing of the Mutant Huntingtin Gene in an In Vitro Model of Huntington’s Disease Nivya Kolli 1,2, Ming Lu 1,2, Panchanan Maiti 1,2,3,4,5, Julien Rossignol 1,2,6 and Gray L. Dunbar 1,2,3,4,* 1 Field Neurosciences Institute laboratory for Restorative Neurology at Central Michigan University,

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Host‐induced gene silencing of multiple genes of

Brief Communication Host-induced gene silencing of multiple genes of Fusarium graminearum enhances resistance to Fusarium head blight in Gravel Minhui Wang1,2,†, Lei Wu3,†, Yuzhen Mei1,2, Youfu Zhao4, Zhonghua Ma1,2,*, Xu Zhang3,* and Yun Chen1,2,* 1Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China

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